Plasmid

Part:BBa_K3779018:Design

Designed by: JiaCheng, Li   Group: iGEM21_NWU-CHINA-B   (2021-06-21)


pPICZαA-SS-bgly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2170
    Illegal XhoI site found at 271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2949
    Illegal NgoMIV site found at 3010
    Illegal AgeI site found at 1889
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 326
    Illegal BsaI.rc site found at 1656
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 2360
    Illegal SapI.rc site found at 989


Design Notes

Codon optimization, gene copy number control


Source

Commercial synthetic

References